detection of lpsa gene in neotyphodium endophytic fungi of grasses in iran

نویسندگان

parisa omoumi

aghafakhr mirlohi

masoud bahar

چکیده

the lpsa gene, a late acting gene in the biosynthetic pathway of ergovaline, a suspected causative agent for fescue toxicosis in cattle, has been cloned from neotyphodium lolii, an endophytic fungus of lolium perenne. in this study, a similar gene was detected in several strains of endophytic neotyphodium spp. isolated from grass hosts endogenous to iran using direct and nested-pcr assays. except for bromus tomentellus, most isolates from other hosts contained this gene. the 747-bp pcr products of the local strains had identical restriction patterns for all tested  restriction enzymes. accordingly, sequence analysis of the nested pcr product amplified from the internal segment of 747-bp band, showed 99% similarity with the corresponding region of the lpsa gene of n. lolii. it therefore appears that prevalence of the lpsa gene with its conserved nature among neotyphodium isolates is mainly host dependent.

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عنوان ژورنال:
iranian journal of biotechnology

ناشر: national institute of genetic engineering and biotechnology

ISSN 1728-3043

دوره 6

شماره 1 2008

کلمات کلیدی

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